Karissa Tilbury


  • University of Wisconsin, 2015, Ph.D., University of Wisconsin, 2011, M.S., University of Wisconsin, 2009, B.S.

Brief biography

The Tilbury Biophotonics Group is interested in developing and applying optical instrumentation to study biological interactions at appropriate spatio-temporal dynamics. Ongoing projects in the lab are focused on the use of Second Harmonic Generation (SHG) microscopy for label-free imaging of collagen in various diseased states.

Research Interests

Biomedical Engineering, Cancer, Extracellular Matrix Remodeling, Cellular Metabolism, Non-linear Microscopy, Second Harmonic Generation (SHG), Multiphoton Microscopy, Spatial Frequency Domain Imaging, STEM Education.


  • Hamilton, J., Breggia, A., Fitzgerald, T., Jones, M., Brooks, P., Tilbury, K.* Khalil, A.* (2022). Multiscale anisotropy analysis of second-harmonic generation collagen imaging of human pancreatic cancer. Frontiers in Oncol. Molecular and Cellular Oncology. vol. 12 at https://www.frontiersin.org/articles/10.3389/fonc.2022.991850(2022
  • Kilroy, E. A., Ignacz, A. C., Brann, K. L., Schaffer, C. E., Varney, D., Alrowaished, S. S., Silknitter, K. J., Miner, J. N., Almaghasilah, A., Spellen, T. L., Lewis, A. D., Tilbury, K., King, B. L., Kelley, J. B., & Henry, C. A. (2022). Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy. ELife, 11, e62760. https://doi.org/10.7554/eLife.62760
  • Tilbury, K., Han, X., Brooks, PC., Khalil, A. (2021). “Multiscale anisotropy analysis of second-harmonic generation collagen imaging of mouse skin.” Journal of Biomedical Optics 26:65002. https://doi.org/10.1117/1.JBO.26.6.065002.
  • Willows, J., Blaszkiewicz, M., Lamore, A., Borer,S., Dubois, A., Garner,E., Breeding, P., Tilbury, K., Khalil, A., Townsend, K. “Visualization and analysis of whole depot adipose tissue neural innervation.” iScience 24:103127. https://doi.org/https://doi.org/10.1016/j.isci.2021.103127
  • Harling, M., Breeding, P., Haysley, T., Chesley, M., Mason M.,Tilbury K “Multiphoton Microscopy for the Characterization of Cellular Behavior on Naturally Derived Polysaccharide Tissue Constructs with Irregular Surfaces for the Development of Platform Biomaterials.” Frontiers in Bioengineering & Biotechnology, 8(802). DOI: 10.3398/fbioe.2020.008002.
  • James, D.S.; Jambor, A.N., Chang, H.Y., Alden, D. Tilbury, K., Sando,N., Campagnola, P. “Probing ECM remodeling in idiopathic pulmonary fibrosis via second harmonic generation microscopy analysis of macro-supramolecular collagen structure.” Journal of Biomedical Optics 2019; 25(1): 1-13. 14505. https://doi.org/10.1117/1.JBO.25.1.014505.
  • Applegate, M., Karrobi, K., Angelo, J. Austin, W., Tabassum, S., Aguenoouno, E., Tilbury, K., Saager R., Gioux S., Robyler, D. “OpenSFDI: An open-source guide for constructing a spatial frequency domain imaging system.” Journal of Biomedical Optics 25(1): 016002. https://doi.org/10.1117/1.JBO.25.1.016002
  • Tilbury KB, Campbell KR, Eliceiri KW, Salih SM, Patankar M, Campagnola, PJ. “Stromal alterations in ovarian cancers via wavelength dependent Second Harmonic Generation microscopy and optical scattering.” BMC Cancer. 2017;6(17). 102. https://doi.org/10.1186/s12885-017-3090-2.
  • Wen B, Campbell K, Tilbury K, Nadiarnykh O, Brewer M, Patankar M, Singh V, Eliceiri K, Campagnola P. “3D texture analysis for classification of second harmonic generation images of human overian cancer.” Scientific Reports. 2016; 6(10). https://doi.org/10.1038/SREP35734
  • Tilbury K, Campagnola, Paul. “Applications of Second Harmonic Generation imaging microscopy in ovarian and breast cancer.” Perspectives in Medicinal Chemistry. 2015;7:21-32. PMC-S13214. doi: 10.4137/PMC.S13214.
  • Chaudhary R, Campbell, KR, Tilbury KB, Vanderby R Jr., Block WF, Kijowski R, Campagnola, PJ. “Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy.” Connect Tissue Res. 2015; 56(2): 76-86. https://doi.org/10.3109/03008207.2015.1013192
  • Tilbury K, Hocker J, Wen BL, Sandbo N, Singh V, Campagnola PJ. “ Second harmonic generation microscopy analysis of extracellular matrix changes in human idiopathic pulmonary fibrosis.” Journal of biomedical optics. 2014;19(8):086014. https://doi.org/10.1117/1.jbo.19.8.086014.
  • Hall G, Tilbury KB, Campbell KR, Eliceiri KW, Campagnola PJ. “Experimental and simulation study of the wavelength dependent second harmonic generation of collage in scattering tissues.” Optic Letter. 2014;39(7):1897-1900. doi: 10.1364/OL.39.001897.
  • Tilbury K, Lien CH, Chen SJ, Campagnola PJ. “Differentiation of Col I and Col III isoforms in stromal models of ovarian cancer by analysis of second harmonic generation polarization and emission directionality.” Biophysical journal. 2014;106(2):354-365. 10.1016/j.bpj.2013.10.044
  • Lien CH, Tilbury K, Chen SJ, Campagnola PJ. “Precise, motion-free polarization control in Second Harmonic Generation microscopy using a liquid crystal modulator in the infinity space.” Biomedical optics express. 2013;4(10):1991-2002. https://doi.org/10.1364/BOE.4.001991.
  • Kapinas K, Lowther KM, Kessler CB, Tilbury K, Lieberman JR, Tirnauer JS, Campagnola P, Delany AM. “Bone matrix osteonectin limits prostate cance cell growth and survivial.” Matrix Biol. 2012;31(5):299-307. doi: 10.1016/j.matbio.2012.03.002.