Basile Tarchini
Education
- PhD., University of Geneva, Switzerland, 2004
Biosketch
My scientific experience is rooted in developmental biology using the mouse as a model system. My graduate project addressed transcriptional regulation in crowded Hox gene complexes and its impact on axial identity, including neuronal organization in the spinal cord and asymmetric digit formation in appendages. My postdoctoral work tackled patterning at the level of a single cell, and identified novel polarity proteins that proved essential for hearing by defining stereocilia placement and elongation in the sensory cells of the inner ear. This project laid the foundation for my independent research program at The Jackson Laboratory, where I have a laboratory since January 2015.
Research Interests
Fundamental to our interaction with the world, hearing and balance require ‘hair cells’ in the inner ear to transduce a mechanical signal (sound, gravity or head movements) into electrical impulses relayed to the brain. Research in our laboratory aims to unravel the developmental mechanisms conferring sensory ability to hair cells. The specialized hair cell compartment dedicated to mechanotransduction, the stereocilia bundle, is produced and oriented by cytoskeleton polarization at the single-cell and tissue level. A molecular understanding of bundle morphogenesis is key to improve our knowledge of hereditary hearing loss, and empowers emerging therapies to regenerate hair cells permanently lost to injury or disease in mammals.
Selected Publications
- Presynaptic Nrxn3 is essential for ribbon-synapse maturation in hair cells. Development. 2024 Oct 1;151(19). doi: 10.1242/dev.202723. Epub 2024 Oct 10. PubMed PMID: 39254120; PubMed Central PMCID: PMC11488651.
- Hartig EI, Day M, Jarysta A, Tarchini B. Proteins required for stereocilia elongation during mammalian hair cell development ensure precise and steady heights during adult life. Proc Natl Acad Sci U S A. 2024 Oct;121(40):e2405455121. doi: 10.1073/pnas.2405455121. Epub 2024 Sep 25. PubMed PMID: 39320919; PubMed Central PMCID: PMC11459194.
- Stanley OR, Swaminathan A, Wojahn E, Bao C, Ahmed ZM, Cullen KE. An open-source tool for automated human-level circling behavior detection. Sci Rep. 2024 Sep 8;14(1):20914. doi: 10.1038/s41598-024-71665-z. PubMed PMID: 39245735; PubMed Central PMCID: PMC11381541.
- Hosur V, Erhardt V, Hartig E, Lorenzo K, Megathlin H, Tarchini B. Large-Scale Genome-Wide Optimization and Prediction of the Cre Recombinase System for Precise Genome Manipulation in Mice. Res Sq. 2024 Jul 3;. doi: 10.21203/rs.3.rs-4595968/v1. PubMed PMID: 39011108; PubMed Central PMCID: PMC11247941.
- Erhardt V, Hartig E, Lorenzo K, Megathlin HR, Tarchini B, Hosur V. Large-Scale Genome-Wide Optimization and Prediction of the Cre Recombinase System for Precise Genome Manipulation in Mice. bioRxiv. 2024 Jun 17;. doi: 10.1101/2024.06.14.599022. PubMed PMID: 38948742; PubMed Central PMCID: PMC11212873.
- Buswinka CJ, Rosenberg DB, Simikyan RG, Osgood RT, Fernandez K, Nitta H, Hayashi Y, Liberman LW, Nguyen E, Yildiz E, Kim J, Jarysta A, Renauld J, Wesson E, Wang H, Thapa P, Bordiga P, McMurtry N, Llamas J, Kitcher SR, López-Porras AI, Cui R, Behnammanesh G, Bird JE, Ballesteros A, Vélez-Ortega AC, Edge ASB, Deans MR, Gnedeva K, Shrestha BR, Manor U, Zhao B, Ricci AJ, Tarchini B, Basch ML, Stepanyan R, Landegger LD, Rutherford MA, Liberman MC, Walters BJ, Kros CJ, Richardson GP, Cunningham LL, Indzhykulian AA. Large-scale annotated dataset for cochlear hair cell detection and classification. Sci Data. 2024 Apr 23;11(1):416. doi: 10.1038/s41597-024-03218-y. PubMed PMID: 38653806; PubMed Central PMCID: PMC11039649.
- Jarysta A, Tadenev ALD, Day M, Krawchuk B, Low BE, Wiles MV, Tarchini B. Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis. Elife. 2024 Apr 23;12. doi: 10.7554/eLife.88186. PubMed PMID: 38651641; PubMed Central PMCID: PMC11037916.
- Voorn RA, Sternbach M, Jarysta A, Rankovic V, Tarchini B, Wolf F, Vogl C. Slow kinesin-dependent microtubular transport facilitates ribbon synapse assembly in developing cochlear inner hair cells. bioRxiv. 2024 Apr 15;. doi: 10.1101/2024.04.12.589153. PubMed PMID: 38659872; PubMed Central PMCID: PMC11042220.
- Jukic A, Lei Z, Cebul ER, Pinter K, Mosqueda N, David S, Tarchini B, Kindt K. Presynaptic Nrxn3 is essential for ribbon-synapse assembly in hair cells. bioRxiv. 2024 Feb 15;. doi: 10.1101/2024.02.14.580267. PubMed PMID: 38410471; PubMed Central PMCID: PMC10896334.
- Jarysta A, Tadenev ALD, Day M, Krawchuk B, Low BE, Wiles MV, Tarchini B. Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis. bioRxiv. 2024 Jan 6;. doi: 10.1101/2023.05.25.542257. PubMed PMID: 37292807; PubMed Central PMCID: PMC10245865.
- Buswinka CJ, Rosenberg DB, Simikyan RG, Osgood RT, Fernandez K, Nitta H, Hayashi Y, Liberman LW, Nguyen E, Yildiz E, Kim J, Jarysta A, Renauld J, Wesson E, Thapa P, Bordiga P, McMurtry N, Llamas J, Kitcher SR, López-Porras AI, Cui R, Behnammanesh G, Bird JE, Ballesteros A, Vélez-Ortega AC, Edge AS, Deans MR, Gnedeva K, Shrestha BR, Manor U, Zhao B, Ricci AJ, Tarchini B, Basch M, Stepanyan RS, Landegger LD, Rutherford M, Liberman MC, Walters BJ, Kros CJ, Richardson GP, Cunningham LL, Indzhykulian AA. Large-scale annotated dataset for cochlear hair cell detection and classification. bioRxiv. 2023 Sep 1;. doi: 10.1101/2023.08.30.553559. PubMed PMID: 37693382; PubMed Central PMCID: PMC10491224.
- Jia S, Ratzan EM, Goodrich EJ, Abrar R, Heiland L, Tarchini B, Deans MR. The dark kinase STK32A regulates hair cell planar polarity opposite of EMX2 in the developing mouse inner ear. Elife. 2023 May 5;12. doi: 10.7554/eLife.84910. PubMed PMID: 37144879; PubMed Central PMCID: PMC10202454.
- Akturk A, Day M, Tarchini B. RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells. Sci Adv. 2022 Oct 21;8(42):eabq2826. doi: 10.1126/sciadv.abq2826. Epub 2022 Oct 19. PubMed PMID: 36260679; PubMed Central PMCID: PMC9581478.
View complete list of publications here.