Steve Murray
Brief Biography
The research in my lab is focused on discovering the developmental mechanisms of morphogenesis and structural birth defects using both forward and reverse genetic approaches. We use innovative CRISPR/Cas9 modeling methods to rapidly investigate the function of novel human mutations associated with these conditions to aid in diagnosis and improve our understanding of disease mechanisms. We take advantage of large-scale mutagenesis programs to identify and study novel single gene knockout developmental phenotypes, and diverse mouse genetic backgrounds to explore the systems-level dynamics of embryonic morphogenesis.
My lab also builds large-scale resources for the broader scientific community including the Knockout Mouse Phenotyping Program (KOMP2), which is part of an international effort the create and phenotype a single gene knockout for every protein coding gene in the mammalian genome. For the JAX Center for Precision Genetics (JCPG), we use genome editing technology to engineer precise models of rare disease and, when feasible, explore genome editing strategies for therapeutic intervention.
Selected Publications
- Valine and Inflammation Drive Epilepsy in a Mouse Model of ECHS1 Deficiency. bioRxiv. 2024 Jun 13;. doi: 10.1101/2024.06.13.598697. PubMed PMID: 38915588; PubMed Central PMCID: PMC11195255.
- Racine JJ, Bachman JF, Zhang JG, Misherghi A, Khadour R, Kaisar S, Bedard O, Jenkins C, Abbott A, Forte E, Rainer P, Rosenthal N, Sattler S, Serreze DV. Murine MHC-Deficient Nonobese Diabetic Mice Carrying Human HLA-DQ8 Develop Severe Myocarditis and Myositis in Response to Anti-PD-1 Immune Checkpoint Inhibitor Cancer Therapy. J Immunol. 2024 Apr 15;212(8):1287-1306. doi: 10.4049/jimmunol.2300841. PubMed PMID: 38426910; PubMed Central PMCID: PMC10984778.
- Qiu C, Martin BK, Welsh IC, Daza RM, Le TM, Huang X, Nichols EK, Taylor ML, Fulton O, O’Day DR, Gomes AR, Ilcisin S, Srivatsan S, Deng X, Disteche CM, Noble WS, Hamazaki N, Moens CB, Kimelman D, Cao J, Schier AF, Spielmann M, Murray SA, Trapnell C, Shendure J. A single-cell time-lapse of mouse prenatal development from gastrula to birth. Nature. 2024 Feb;626(8001):1084-1093. doi: 10.1038/s41586-024-07069-w. Epub 2024 Feb 14. PubMed PMID: 38355799; PubMed Central PMCID: PMC10901739.
- Roy TA, Bubier JA, Dickson PE, Wilcox TD, Ndukum J, Clark JW, Sukoff Rizzo SJ, Crabbe JC, Denegre JM, Svenson KL, Braun RE, Kumar V, Murray SA, White JK, Philip VM, Chesler EJ. Discovery and validation of genes driving drug-intake and related behavioral traits in mice. Genes Brain Behav. 2024 Jan 2;23(1):e12875. doi: 10.1111/gbb.12875. [Epub ahead of print] PubMed PMID: 38164795; PubMed Central PMCID: PMC10780947.
- Racine JJ, Misherghi A, Dwyer JR, Maser R, Forte E, Bedard O, Sattler S, Pugliese A, Landry L, Elso C, Nakayama M, Mannering S, Rosenthal N, Serreze DV. HLA-DQ8 Supports Development of Insulitis Mediated by Insulin-Reactive Human TCR-Transgenic T Cells in Nonobese Diabetic Mice. J Immunol. 2023 Dec 15;211(12):1792-1805. doi: 10.4049/jimmunol.2300303. PubMed PMID: 37877672; PubMed Central PMCID: PMC10939972.
- Luzzio A, Edie S, Palmer K, Caddle LB, Urban R, Goodwin LO, Welsh IC, Reinholdt LG, Bergstrom DE, Cox TC, Donahue LR, Murray SA. The spontaneous mouse mutant low set ears (Lse) is caused by tandem duplication of Fgf3 and Fgf4. Mamm Genome. 2023 Sep;34(3):453-463. doi: 10.1007/s00335-023-09999-8. Epub 2023 Jun 21. PubMed PMID: 37341808.
- Lao YH, Ji R, Zhou JK, Snow KJ, Kwon N, Saville E, He S, Chauhan S, Chi CW, Datta MS, Zhang H, Quek CH, Cai SS, Li M, Gaitan Y, Bechtel L, Wu SY, Lutz CM, Tomer R, Murray SA, Chavez A, Konofagou EE, Leong KW. Focused ultrasound-mediated brain genome editing. Proc Natl Acad Sci U S A. 2023 Aug 22;120(34):e2302910120. doi: 10.1073/pnas.2302910120. Epub 2023 Aug 14. PubMed PMID: 37579143; PubMed Central PMCID: PMC10450663.
- Peterson KA, Khalouei S, Hanafi N, Wood JA, Lanza DG, Lintott LG, Willis BJ, Seavitt JR, Braun RE, Dickinson ME, White JK, Lloyd KCK, Heaney JD, Murray SA, Ramani A, Nutter LMJ. Whole genome analysis for 163 gRNAs in Cas9-edited mice reveals minimal off-target activity. Commun Biol. 2023 Jun 10;6(1):626. doi: 10.1038/s42003-023-04974-0. PubMed PMID: 37301944; PubMed Central PMCID: PMC10257658.
- Oestereicher MA, Wotton JM, Ayabe S, Bou About G, Cheng TK, Choi JH, Clary D, Dew EM, Elfertak L, Guimond A, Haseli Mashhadi H, Heaney JD, Kelsey L, Keskivali-Bond P, Lopez Gomez F, Marschall S, McFarland M, Meziane H, Munoz Fuentes V, Nam KH, Nichtová Z, Pimm D, Bower L, Prochazka J, Rozman J, Santos L, Stewart M, Tanaka N, Ward CS, Willett AME, Wilson R, Braun RE, Dickinson ME, Flenniken AM, Herault Y, Lloyd KCK, Mallon AM, McKerlie C, Murray SA, Nutter LMJ, Sedlacek R, Seong JK, Sorg T, Tamura M, Wells S, Schneltzer E, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, White JK, Spielmann N. Comprehensive ECG reference intervals in C57BL/6N substrains provide a generalizable guide for cardiac electrophysiology studies in mice. Mamm Genome. 2023 Jun;34(2):180-199. doi: 10.1007/s00335-023-09995-y. Epub 2023 Jun 9. Review. PubMed PMID: 37294348; PubMed Central PMCID: PMC10290602.
